Mutation Analysis of the BRCA1 and BRCA2 Genes in the Belgian Patient Population and Identification of a Belgian Founder Mutation BRCA1 IVS5+3A>G

Article type: Research Article

Authors: Claes, Kathleen | Machackova, Eva | De Vos, Michel | Poppe, Bruce | De Paepe, Anne | Messiaen, Ludwine

Affiliations: Department of Medical Genetics, University Hospital Gent (0K5), De Pintelaan 185, B-9000 Gent, Belgium

Note: [] Correspondence: Ludwine Messiaen, PhD, Department of Medical Genetics, University Hospital Gent (0K5), De Pintelaan 185, B-9000 Gent, Belgium, Tel.: +32 9240 2478, Fax: +32 9240 4970, E-mail: [email protected]

Abstract: Since the identification of the BRCA1 and BRCA2 genes, several hundred different germline mutations in both genes have been reported.  Recurrent mutations are rare and mainly due to founder effects.  As the mutational spectrum of the BRCA1 and BRCA2 genes in the Belgian patient population is largely unknown, we initiated mutation analysis for the complete coding sequence of both genes in Belgian families with multiple breast and/or ovarian cancer patients and in "sporadic" patients with early onset disease.  We completed the analysis in 49 families and in 19 "sporadic" female patients with early onset breast and/or ovarian cancer.  In 15 families we identified a mutation (12 mutations in BRCA1 and 3 mutations in BRCA2).  In 5 apparently unrelated families the same splice site mutation was identified (BRCA1 IVS5+3A>G).  Haplotype analysis revealed a common haplotype immediately flanking the mutation in all families suggesting that disease alleles are identical by descent.  In none of the 19 sporadic patients was a mutation found.

Journal: Disease Markers, vol. 15, no. 1-3, pp. 69-73, 1999