Affiliations: [a] Department of Orthopedics, the Affiliated Hospital of Qinghai University, Xining, Qinghai, China | [b] Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu, China
Correspondence:
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Corresponding author: Dongtang Yuan, Department of Orthopedics, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, China. Tel.: +86 517 84952309; Fax: +86 517 84952309; E-mail:[email protected]
Abstract: BACKGROUND: MicroRNA (miR)-191 has been observed to be
overexpressed in osteosarcoma cell lines in comparison with osteoblasts. OBJECTIVE: To investigate the clinical significance of miR-191 in
human osteosarcomas. METHODS: Quantitative PCR was performed to detect miR-191
expression in osteosarcoma tissues and patients' sera. RESULTS: miR-191 expression levels, both in osteosarcoma tissues
and patients' sera, were significantly higher than those in matched adjacent
normal bone tissues and healthy controls (both P< 0.001). Importantly,
miR-191 could efficiently screen osteosarcoma patients from healthy controls
(Area under receiver operating characteristic curve, AUC = 0.808). Then, high
serum miR-191 expression was significantly associated with advanced clinical
stage (P= 0.001), large tumor size (P= 0.01) and positive distant metastasis
(P= 0.001). Moreover, overall and disease-free survival durations
in patients with high miR-191 expression were both shorter than those with
low miR-191 expression. Multivariate analysis further
identified serum miR-191 level as an independent and significant
prognostic factor for both overall survival (P= 0.01) and
disease-free survival (P= 0.02). CONCLUSIONS: Our data provide new insights for the involvement of
miR-191 in osteosarcoma and suggest that the increased expression of miR-191
may be associated with aggressive tumor progression and adverse outcome. Of
note, serum miR-191 quantification may be a promising biomarker for the
diagnosis and prognosis in osteosarcoma.
