Affiliations: Department of Neurosurgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Correspondence:
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Corresponding authors: Huoniu Ouyang and Zhilin Guo, Department of Neurosurgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Rd, Shanghai 200011, China. Tel.: +86 21 2327 1699 Ext.5155; Fax: +86 21 6313 6856;
E-mail: [email protected],[email protected]
Abstract:
BACKGROUND: Glioma is one of the most common primary malignant
brain tumors. However, the potential molecular mechanism of glioma
tumorigenesis is limited. In this study, we aimed to investigate the
functional relationship between glioma and a potential tumor related gene
JUB. METHODS: Lentivirus-based RNA interference was carried out to knock
down JUB expression in human glioma cells U251. The effects of
JUB on cell proliferation and cell cycle were detected by MTT,
colony formation and flow cytometry assays. RESULTS: Lentivirus-mediated shRNA could effectively suppress JUB
expression in U251 cells, resulting in significant decreases in cell
proliferation and colony formation. Flow cytometry analysis showed that
JUB silencing blocked cell cycle at S and G2/M phases, and induced apoptosis,
which could contribute to cell growth suppression. Furthermore, knockdown of
JUB caused downregulation of CDK6 and activations of Caspase 3 and PARP. CONCLUSIONS: The results in this study uncovered that JUB was a
regulator involved in proliferation of glioma cells, and it could be used as
a potential therapeutic target for glioma.
Keywords: Glioblastomas, JUB, proliferation, RNA interference, ajuba LIM protein
