Affiliations: [a] Department of Pathogenic Biology, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China | [b] Department of Clinical Laboratory, Zibo Central Hospital, Zibo, Shandong, China | [c] Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, Shandong, China
Correspondence:
[*]
Corresponding author: Bing Luo, Department of Pathogeny Biology, School of Basic Medicine, Qingdao University, No. 308 Ningxia Road, Qingdao 266071, Shandong, China. E-mail: [email protected].
Note: [1] These authors contributed equally.
Abstract: BACKGROUND AND OBJECTIVE: EBV-associated gastric cancer (EBVaGC) is a distinct subtype of GC, and EBV plays an important role in tumor progress. The standard method to identify EBV-positive tumor is determined by in situ hybridization for EBV-encoded EBERs in tumor tissues. The present study aims to detect the serological expression of EBV-related antibodies and ET-1 axis to provide a noninvasive method for diagnosis of EBVaGC. METHODS: The content of EBV-related antibodies and ET-1 axis in preoperative peripheral blood of GC was performed by Chemiluminescence and ELISA assay. The EBV DNA copy number was measured by qRT-PCR. RESULTS: The results showed that the levels of anti-EBV early antigen (EA) IgG, viral capsid antigen (VCA) IgA, nuclear antigen (NA) IgG, and EBV DNA copy number were significantly higher in EBVaGC. The ET-1 axis level was much lower in EBVaGC than EBVnGC. CONCLUSIONS: The combined detection of specific anti-EBV antibodies and ET-1 axis might provide new molecular markers for the identification of EBVaGC.
Keywords: EBV-related antibodies, ET-1, ETAR, ETBR, DNA copy number
