Plasma exosome-derived microRNA-532 as a novel predictor for acute myeloid leukemia

Article type: Research Article

Authors: Lin, Xia^{b; 1} | Ling, Qing^{b; 1} | Lv, Yunfei^{b; 1} | Ye, Wenle^b | Huang, Jiansong^{b; c} | Li, Xia^b | Guo, Qi^d | Wang, Jinghan^{a; b; c; *} | Li, Zhongqi^{e; *} | Jin, Jie^{a; b; c; *}

Affiliations: [a] Department of Hematology, The First Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, Zhejiang, China | [b] Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang, China | [c] Key Laboratory of Hematologic Malignancies, Diagnosis and Treatment, Hangzhou, Zhejiang, China | [d] Department of Nephrology, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, China | [e] The Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, China

Correspondence: [*] Corresponding authors: Jinghan Wang and Jie Jin, Department of Hematology, The First Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou, Zhejiang, China. Tel.: +86 571 87236898; Fax: +86 571 87236702; E-mail: [email protected] or [email protected]; Zhongqi Li, The Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang, China. E-mail: [email protected].

Note: [1] Xia Lin, Qing Ling, and Yunfei Lv contributed equally to this work.

Abstract: BACKGROUND: The interest in plasma biomarkers has increased recently. Plasma exosome-derived microRNA-532 is aberrantly expressed in a variety of human cancers and has the prognostic value in many solid tumors. However, the prognostic impact of the expression value on AML remains unclear. OBJECTIVE: The aim of this study is to investigate the prognostic value of exosome-derived microRNA-532 in AML patients. METHODS: We performed the real-time PCR to quantify exosome-derived microRNA-532 in plasma of 198 AML patients. To assess the prognostic value, we performed Cox regression analyses in the context of well-established clinical and molecular markers. Cellular metabolic profile was conducted to help us understand the biological insight of its expression. RESULTS: The expression level was not associated with white blood cell counts, age, FAB subtypes, cytogenetic risk groups and genes of FLT3-ITD, NPM1, CEBPA and DNMT3A mutations. Interestingly, high expressers had a favorable overall survival in the univariate analysis. This prognostic value was testified in the multivariate analysis. Moreover, up-regulation of miR-532 was negatively associated with cellular energy like fructose and glutamine. CONCLUSION: We found plasma exosome-derived microRNA-532 can be used as a novel survival predictor for acute myeloid leukemia.

Keywords: Acute myeloid leukemia, exosome, microRNAs

DOI: 10.3233/CBM-191164

Journal: Cancer Biomarkers, vol. 28, no. 2, pp. 151-158, 2020