Affiliations: [a] Institute of Bioinformatics, International Technology Park, Bangalore, India | [b] Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, India | [c] Department of Surgical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, India | [d] Department of Pathology, Kidwai Memorial Institute of Oncology, Bangalore, India | [e] Department of Biochemistry, Central Food Technological Research Institute, Mysore, India | [f] Cancer Science Institute of Singapore, National University of Singapore, Singapore | [g] Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India
Correspondence:
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Corresponding author: Prashant Kumar, Faculty Scientist, %****␣cbm-25-cbm190013_temp.tex␣Line␣25␣**** Institute of Bioinformatics, 7 th floor, Discoverer building, International Technology Park, Bangalore 560 066, India. E-mail: [email protected].
Abstract: BACKGROUND: Transcription factors are commonly deregulated in various cancers. Here, we evaluated role of ELF3 in pathogenesis of bladder carcinoma (BCa). MATERIALS AND METHODS: We confirmed ELF3 expression pattern in BCa cell lines using western blot; and in different grades of tumors using Immunohistochemistry. Cell invasion assay was employed to demonstrate potential role of ELF3 in EMT. RESULTS AND CONCLUSION: ELF3 showed selective expression in low-grade cell lines and tumor tissues. Overexpression of ELF3 in mesenchymal cell line UMUC3 resulted in reduced invasion and decreased expression of mesenchymal markers. We observed association of low ELF3 expression with increased risk and overall poor survival using publicly available data. ELF3-modulated reversal of EMT might be a useful strategy in the treatment of bladder cancer.
