Affiliations: [a] The First Department of Orthopedics, Qingdao West Coast New Area Central Hospital, Qingdao, Shandong, China | [b] Outpatient Department, Yantai Yeda Hospital, Yantai, Shandong, China | [c] Department of Internal Medicine Ward, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [d] Department of Anesthesiology, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [e] Department of Blood Transfusion, The People’s Hospital of Zhangqiu Area, Jinan, Shandong, China | [f] Department of Oncology, Jining No. 1 People’s Hospital, Jining, Shandong, China
Note: [1] These authors contributed equally to this study and share first Authorship.
Abstract: OBJECTIVE: Osteosarcoma is the most common malignant tumor of bone with high recurrent rate. miR-486 was downregulated and acted as a tumor suppressor in plenty of tumors. The purpose of this study was to explore how miR-486 worked in osteosarcoma on cell invasion and EMT. RESULTS: miR-486 was low expressed in osteosarcoma while PIM1 was overexpressed, and it had negative correlation between miR-486 and PIM1. miR-486 upregulation or PIM1 downregulation could inhibit osteosarcoma cell invasion and EMT. Meanwhile, miR-486 mediated PIM1 expression through binding to PIM1 mRNA 3’-UTR. PIM1 could reveal partial function of miR-486 on osteosarcoma invasion. In addition, miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcoma patients. CONCLUSION: miR-486 regulated osteosarcoma cell invasion and EMT through targeting to PIM1. miR-486 low expression or PIM1 overexpression predicted poor prognosis of osteosarcoma patients. The newly identified miR-486/PIM1 axis provides novel insight into the pathogenesis of osteosarcoma.
