The KISS1 gene overexpression as a potential molecular marker for cervical cancer cells

Article type: Research Article

Authors: Taniguchi-Ponciano, Keiko^{a; b} | Ribas-Aparicio, Rosa María^b | Marrero-Rodríguez, Daniel^{a; c} | Arreola-De la Cruz, Hugo^a | Huerta-Padilla, Víctor^a | Muñoz, Nancy^a | Gómez-Ortiz, Laura^a | Ponce-Navarrete, Gustavo^a | Rodríguez-Esquivel, Miriam^a | Mendoza-Rodríguez, Mónica^{a; d} | Gómez-Virgilio, Laura^c | Peralta, Raúl^e | Serna, Luis^f | Gómez, Guillermo^f | Ortiz, Jorge^f | Mantilla, Alejandra^g | Hernández, Daniel^h | Hernández, Ángeles^h | Bandala, Cindyⁱ | Salcedo, Mauricio^{a; *}

Affiliations: [a] Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI, IMSS, Mexico City, Mexico | [b] Programa de Doctorado en Ciencias en Biomedicina y Biotecnología Molecular, Departamento de Microbiología, Escuela de Ciencias Biológicas Instituto Politécnico Nacional (ENCB-IPN), Mexico City, Mexico | [c] Laboratorio 5 Departamento de Biomedicina Molecular, CINVESTAV-IPN, Mexico City, Mexico | [d] Universidad Politécnico de Huatusco, Huatusco, Veracruz, Mexico | [e] Centro de Investigación en Dinámica Celular, Universidad Autónoma del Estado de Morelos, Cuernavaca, Mexico | [f] Clínica de Displasias, Hospital General de México, Mexico City, Mexico | [g] Servicio de Patología, Hospital de Oncología CMN-SXXI, IMSS, Mexico City, Mexico | [h] División de Laboratorios de Vigilancia e Investigación Epidemiológica, IMSS, Mexico City, Mexico | [i] Unidad de Apoyo a la Investigación, Instituto Nacional de Rehabilitación, Secretaría de Salud, Mexico City, Mexico

Correspondence: [*] Corresponding author: Mauricio Salcedo, Instituto Mexicano del Seguro Social, Centro Médico Nacional Siglo XXI, Hospital de Oncología, Unidad de Investigación Médica en Enfermedades Oncológicas, Av. Cuauhtémoc 330, Col. Doctores, México D.F., México 06720, Mexico. Tel.: +52 56277900 Ext. 22706; E-mail: [email protected].

Abstract: BACKGROUND: Similarities between the pathologic progression of cancer and the physiologic process of placentation have been recognized for many years proposing that both present similar mechanisms and processes. Cervical cancer (CC) is one of the most frequent neoplasia among Mexican women turning it into an important health problem. OBJECTIVE: The aim of this study was to determine the degree of the involvement of pregnancy related genes and in cancer progression by in-silico analysis and validated in CC samples. RESULTS: The data mining analysis resulted in the identification of genes expressed in term placenta, first trimester placenta and normal cervical tissues. Finally, we selected KISS1 for the involvement of pregnancy related gene and also in cancer process. In order to explore KISS1 in CC, we analyzed Copy Number Variation (CNV) and gene expression using microarray experiments. KISS1 showed 20% genomic gain in 1q32.1 on CC samples. Furthermore, microarray analysis showed KISS1 as up-regulated genes. Results were validated showing an overexpression of 85% of KISS1 in CC samples. CONCLUSIONS: Data suggest KISS1 as a great candidate for CC molecular markers or as a therapeutic target for CC. Also, HPV presence does not seem to alter the KISS1 expression in CC.

Keywords: KISS1, cervical cancer, in-silico analysis, molecular marker

DOI: 10.3233/CBM-181215

Journal: Cancer Biomarkers, vol. 22, no. 4, pp. 709-719, 2018