MicroRNA-186 suppresses lung cancer progression by targeting SIRT6

Article type: Research Article

Authors: Ruan, Libo^{a; b; c} | Chen, Jun^b | Ruan, Litao^d | Yang, Tianrui^b | Wang, Ping^{a; c; e; *}

Affiliations: [a] Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Yunnan, China | [b] Department of Geriatric Medicine, The First People’s Hospital of Yunnan Province, Yunnan, China | [c] Medical School of Kunming University of Science and Technology, Yunnan, China | [d] Institute of Medical Biology, Chinese Academy of Medical Sciences, Yunnan, China | [e] Department of Thoracic Surgery, The First People’s Hospital of Yunnan Province, Yunnan, China

Correspondence: [*] Corresponding author: Ping Wang, No.157 Jinbi Road, Xishan District, Kunming 650034, Yunnan, China. E-mail: [email protected].

Abstract: MicroRNAs are a class of small non-coding RNA molecules that play crucial roles in the initiation and progression of lung cancer. This study was undertaken to investigate the expression and roles of miR-186 in lung cancer. Ectopic expression experiments demonstrated that miR-186 functions as a tumor suppressor. Bioinformatic predictions, a luciferase reporter assay, and protein expression analysis suggested that miR-186 could inhibit the protein levels of SIRT6, a purported tumor suppressor gene. Collectively, our results indicated that miR-186 could inhibit lung cancer progression through targeting SIRT6 and that miR-186 may be a therapeutic target for lung cancer.

Keywords: Lung cancer, microRNA, miR-186, SIRT6, apoptosis

DOI: 10.3233/CBM-170650

Journal: Cancer Biomarkers, vol. 21, no. 2, pp. 415-423, 2018