Affiliations: [a] Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China | [b] Department of Internal Medicine, Harbin Red Cross Central Hospital, Harbin, Heilongjiang, China | [c] Department of Sixth Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China | [d] Department of Internal Medicine, The First Hospital of Harbin, Harbin, Heilongjiang, China
Correspondence:
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Corresponding author: Yan Yu, Department of Sixth Internal Medicine, Harbin Medical University Cancer Hospital, No. 150, Haping Road, Nangang District, Harbin 150040, Heilongjiang, China. Tel.:/Fax: +86 0451 86298000; E-mail: [email protected].
Abstract: Our study aimed to explore the effects of long noncoding RNA (lncRNA)-ANCR on the invasion and migration of colorectal cancer (CRC) cells by regulating enhancer of zeste homolog 2 (EZH2) expression. CRC tissues and adjacent normal tissues were collected and CRC SW620 cells line and normal human intestinal epithelial cells (HIECs) were incubated. CRC SW620 cells line was transfected with ANCR-siRNA. The expressions of ANCR and EZH2 mRNA were measured by real-time quantitative polymerase chain reaction (RT-qPCR). EZH2 and trimethylation of H3K27 (H3K27me3) protein expressions were detected using Western blotting. The relationship between ANCR and EZH2 was determined through RNA pull-down and co-immunoprecipitation (co-IP) assays. Cell invasion and migration were determined by Trans-well and cell scratch assays. ANCR, EZH2 and H3K27me3 expressions were up-regulated in CRC tissues and SW620 cells (all P< 0.05). After transfected with ANCR-siRNA, SW620 cells showed decreased ANCR expression and EZH2 mRNA and protein expressions (all P< 0.05). According to the results of RNA pull-down and co-IP assays, ANCR could specifically bind to EZH2. The results of Trans-well and cell scratch tests showed that when ANCR expression was decreased, the invasion and migration abilities of SW620 cells significantly declined (both P< 0.05). In conclusion, these results suggest that lncRNA-ANCR could influence the invasion and migration of CRC cells by specifically binding to EZH2.
Keywords: LncRNA, ANCR, EZH2, invasion, migration, colorectal cancer
