Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Fantony, Joseph J.a | Longo, Thomas A.a | Gopalakrishna, Ajaya | Owusu, Richmonda | Lance, Raymond S.c | Foo, Wen-Chid | Inman, Brant A.a | Abern, Michael R.b; *
Affiliations: [a] Division of Urology, Duke University Medical Center, Durham, NC, USA | [b] Department of Urology, University of Illinois Chicago, Chicago, IL, USA | [c] Division of Urology, Urology of Virginia, Hampton, VA, USA | [d] Department of Pathology, Duke University Medical Center, Durham, NC, USA
Correspondence: [*] Corresponding author: Michael R. Abern, Department of Urology, University of Illinois Chicago, 820 South Wood Street Suite 515, Chicago, IL 60612, USA. Tel.: +1 312 996 9330; E-mail:[email protected]
Abstract: BACKGROUND: Abnormal methylation of urinary TWIST1 and NID2 conferred high sensitivity and specificity for the detection of urothelial carcinoma. OBJECTIVE: We examine the performance of the urine-based TWIST1/NID2 methylation assay with the addition of urine cytology for the detection of urothelial carcinoma. MATERIALS AND METHODS: A prospective multi-institutional study was conducted to assess the performance of a methylation assay for patients with hematuria or under surveillance for non-muscle invasive bladder cancer (NMIBC). All patients underwent cystoscopy, a methylation assay, and cytology. Receiver operator characteristic (ROC) curves were constructed for cytology alone, the methylation assay alone, and a combined model. Areas under the curve (AUC) were compared using likelihood ratio tests. RESULTS: A total of 172 patients were enrolled (37% for hematuria and 63% NMIBC). The AUC for cytology alone with equivocal cytologies positive was 0.704, and improved to 0.773 with the addition of the DNA methylation assay (p < 0.001). When the equivocal cytologies were considered negative, the AUC improved from 0.558 to 0.697 with the addition of the DNA methylation assay (p = 0.003). CONCLUSIONS: Addition of a TWIST1/NID2-based DNA methylation assay adds diagnostic value to urine cytology and the model is sensitive to the classification of equivocal cytology.
Keywords: Diagnostic test, epigenetic, sensitivity, specificity, urothelial carcinoma, smoking
DOI: 10.3233/CBM-160261
Journal: Cancer Biomarkers, vol. 18, no. 4, pp. 381-387, 2017
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]