Fetal alcohol spectrum disorders (FASDs) are associated with a wide range of cognitive deficiencies.
We previously found that gestational exposure to moderate levels of alcohol in mice throughout the 1st-2nd human trimester-equivalents for brain development results in profound impairment of the hippocampal neurogenic response to enriched environment (EE) in adulthood, without altering baseline neurogenesis rate under standard housing (SH). However, the functional and structural consequences of impaired EE-mediated neurogenesis in the context of prenatal alcohol exposure (PAE) have not been determined.
Here, we demonstrate that PAE-EE mice display impaired performance on a neurogenesis-dependent pattern discrimination task, broadened behavioral activation of the dentate gyrus, as assessed by expression of the immediate early gene, c-Fos, and impaired dendritic branching of adult-generated dentate granule cells (aDGCs).
These studies further underscore the impact of moderate gestational alcohol exposure on adult hippocampal plasticity and support adult hippocampal neurogenesis as a potential therapeutic target to remediate certain neurological outcomes in FASD.
Fetal alcohol spectrum disorders (FASD) are the leading preventable cause of intellectual and neurodevelopmental disability, and impose considerable financial and economic burden on societies . The prevalence of FASD among school-age children within the USA has been estimated at ∼2.5% . A recent CDC report indicates that approximately 1 in 10 women consume alcohol at some point during pregnancy , yet few interventions are available for mitigating the cognitive and neurobehavioral disabilities associated with FASD . Evidence from clinical and preclinical FASD research has shown that gestational exposure to even moderate levels of alcohol can result in impairment of brain function . Although prenatal alcohol exposure (PAE) affects many brain regions, structural and functional impairments of the hippocampus are particularly well documented [6–9].
Adult hippocampal neurogenesis represents a unique form of neural plasticity that promotes flexible learning and adaptive behavioral responses to cognitive and emotional challenge [10–15], which are aspects of mental function often disrupted in FASD [16–19]. Our laboratory first reported a negative impact of moderate PAE on adult hippocampal neurogenesis in mice 20]. Using both continuous and limited access voluntary drinking paradigms to model moderate PAE (maternal blood alcohol concentrations ∼80–120 mg/dl), and genetic labeling methods to identify the neurogenic lineage, we demonstrated that although gestational exposure to moderate levels of alcohol had no effect on adult neurogenesis under standard housing conditions, it resulted in severe impairment of the neurogenic response to enriched environment (EE) [20–22]. These findings suggest that even moderate gestational alcohol exposure might also impair the ability of FASD individuals to respond optimally to human correlates of EE, or to respond optimally to behavioral training/interventions known to improve hippocampal-associated behaviors in humans [23, 24]. This is relevant given that moderate alcohol consumption is the most common pattern of consumption among women who report drinking alcohol during pregnancy .
Taken together, these observations suggest that restoring EE-mediated postnatal hippocampal neurogenesis may improve certain neurological outcomes associated with moderate gestational alcohol exposure. Validation of this hypothesis, however, requires the ability to pinpoint functional consequences of impaired EE-mediated neurogenesis within the context of PAE, since PAE impairs many other aspects of hippocampal plasticity. In the current study, we sought to elucidate the behavioral consequences of impaired EE-mediated neurogenesis in PAE mice using a neurogenesis-dependent A-B contextual discrimination task, and to determine whether impairment of EE-mediated neurogenesis in PAE mice is associated with alterations in hippocampal network activation and/or dendritic maturation of adult-generated DGCs (aDGCs), which could mediate these behavioral deficits.
MATERIALS AND METHODS
C57Bl6/J or Nestin-CreERT2: tdTomato bitransgenic mice were used for these studies. C57Bl6/J mice were purchased from Jackson Laboratories. Nestin-CreERT2: tdTomato bitransgenic mice were obtained from breeding colonies maintained in the University of New Mexico Health Sciences Center Animal Facility. For transgenic mice, breeding colonies were maintained for homozygosity at both the Nestin-CreERT2  and Ai9 (RCL-tdT) transgene loci , on a C57BL/6J genetic background as previously described [21, 28]. Genotyping was routinely performed by PCR analysis of tail genomic DNA as previously described in detail . All mice were housed under reverse 12-hr dark/12-hr light cycle (lights off at 08:00 h) in a humidity and temperature controlled room with food and water available ad libitum. Animal procedures were approved by the University of New Mexico Institutional Animal Care and Use Committee in accordance with NIH policies on Humane Care and Use of Laboratory Animals.
Prenatal alcohol exposure (PAE)
PAE offspring were generated within the New Mexico Alcohol Research Center Scientific Core, using a well-characterized limited-access “drinking-in-the-dark” gestational ethanol exposure paradigm as previously described in detail . Food and water consumption, maternal weight gain, litter size, pup weight, pup retrieval times and time on nest does not differ between the alcohol-exposed and control animals in this drinking paradigm . Briefly, 60 day old C57Bl6/J or Nestin-CreERT2:tdTomato female mice were offered a 0.066% saccharin solution containing EtOH, instead of water, for 4 hrs per day from 10:00–14:00. (note: C57Bl/6J mice were used for c-Fos experiments, whereas Nestin-CreERT2:tdTomato transgenic mice were used for all other experiments). Following a 5 day gradual ramp-up period (from 0–10% EtOH), the mice were subsequently maintained on a 10% EtOH drinking regimen for 2 weeks prior to pregnancy and throughout gestation. Female mice offered 0.066% saccharin (SAC) without EtOH during the same periods served as controls. Consumption volume during the 4 hr access period, as determined for dams beginning after one week of drinking 10% alcohol, was 5.74 ± 0.41 gm/kg, n = 22 (range 3.46 – 10.90 gm/kg; median 4.82 gm/kg). In this paradigm, blood alcohol concentrations directly correlate to the average amount of ethanol consumed over the 4 hr drinking period throughout gestation . Based on this correlation, average daily maternal blood alcohol concentrations (BACs) were estimated to be 80–90 mg/dL throughout gestation.
Tamoxifen (TAM; Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 10% EtOH/90% sunflower oil (Sigma-Aldrich) and administered by intraperitoneal injections (180 mg/kg) to Nestin-CreERT2:tdTomato bitransgenic mice (SAC and PAE offspring) for five consecutive days for quantification of aDGCs as previously described [21, 22, 28] or as a single injection (180 mg/kg) for dendritic morphological analysis at the ages indicated in text.
Standard housing (SH) and Enriched environment (EE)
PAE and SAC offspring were gender segregated at weaning and housed thereafter in standard housing (SH) or enriched environment (EE) conditions until sacrifice. SH comprised a standard mouse cage (28 cm×18 cm×13 cm) without running wheels or toys (three mice/cage). EE included a larger cage (48 cm×27 cm×20 cm) containing two running wheels and multiple objects (ladder, tunnel and hanging toys that were changed out weekly; six mice/cage) as previously described [20, 22].
A-B Contextual-fear discrimination
Behavioral testing was performed in the University of New Mexico Center for Brain Recovery and Repair Preclinical Core Facility using a protocol blinded to treatment. Mice were trained on the A-B contextual-fear discrimination learning task as previously described [22, 30, 31], and originally modified from Sahay et al.,  (Fig. 1A and 1B). This behavioral assay tests the cognitive ability of mice to learn to discriminate between two similar contexts, using a protocol that is dependent upon the activity of aDGCs [24, 31–33]. All experiments were performed using the automated NIR Video Fear Conditioning System for Mouse (Med Associates, Fairfax, VT). Briefly, Context A (shock condition) was comprised of a standard chamber with a stainless steel floor with steel grid rods arranged in a straight horizontal plane, clear Plexiglas front wall, and aluminum side and back walls. A similar chamber comprised Context B (non-shock), except for a non-shock floor comprised of stainless steel grid rods arranged in 2 offset horizontal planes, and side and back walls covered in striped black and white contact paper. All mice were habituated in a red-light holding room for one hour before the first training session and were returned to their home cages in the animal facility following the final training session each day. Each mouse was individually placed into either Context A (foot shock) or Context B (no foot shock) for 90 s, followed immediately by foot shock (0.5 mA) for 2 s in Context A only. Following a 90 s interval, mice in Context A received a second foot shock and were removed from the chamber 30 s later. Mice in Context B received no shock or other aversive stimulus during the trial and remained within the chamber for the same period of time. Three hours following the first session, mice were exposed to a second identical training session, except that the mouse initially subjected to Context A (shock) was subjected to Context B (no shock), and vice versa. This sequence was repeated once per day for 7 consecutive days, with the order of testing in Context A vs. Context B reversed every day for each individual mouse. Freezing time was measured with an automated software system (Video Fear Conditioning “Video Freeze®” Software, Med Associate, Fairfax, VT) for analysis of digitally-recorded motion (30 frames per second) under red-light conditions. Freezing was defined using a motion threshold of 10 and minimum freeze duration of 30 frames (i.e., subject must remain immobile for one second to be registered as a freeze). A discrimination score was automatically calculated for each mouse for each day of training [(freezing score Context A - freezing score Context B)/(freezing score Context A + freezing score Context B)], during the first 90 s within either chamber. Thus, higher discrimination scores indicate better contextual discrimination. All mice were returned to home cages (EE or SH) and sacrificed 1 week later (∼11 weeks post-tamoxifen and ∼4.0 months of age) and brains from 4 mice per experimental group were randomly selected for histological quantification of tdTomato+ aDGCs as described below.
Exposure to novel environment
Mice were transferred in their home cage to a nearby behavioral testing room and acclimated for one hour before exposure to novel environment. Each mouse was individually removed from its home cage and placed into an open field novel environment for 30 minutes, followed by return to home cage for 80 minutes prior to sacrifice for c-Fos expression analysis. All experiments were performed under infrared lighting conditions during the reversed light/dark cycle. Control mice not exposed to novel environment remained within their home cages in their home room undisturbed until sacrifice.
All mice were overdosed with sodium pentobarbital (150 mg/kg, Fort Dodge Animal Health, Fort Dodge, IA) administered by intraperitoneal injection, and transcardially perfused with phosphate-buffered saline (PBS) containing 0.1% procaine and 2 U/ml heparin, followed by 4% paraformaldehyde (w/v) in 0.1 M PBS. The brains were post-fixed overnight, cryoprotected with 30% sucrose (w/v) in 0.1 M PBS for 48 hours at 4°C and sectioned in the coronal plane at 40μm (quantification of cell numbers) or 100μm (dendritic morphology analysis) as indicated in text, using a freezing sliding knife mictrotome. Free-floating tissue sections were immunostained using primary antibodies directed against the neuronal nuclear antigen, NeuN (1:1000, EMD Millipore, MAB 377) or against the immediate early gene, c-Fos (1:1000, Calbiochem, PC-38T) and visualized using FITC-conjugated or Cy3-conjugated secondary antibodies (1:250; Jackson Immunoresearch Laboratories, Westgrove, PA), respectively. Where indicated, sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific, Carlsbad, CA). All sections were mounted onto glass slides and cover slipped using Fluoromount G mounting media (Electron Microscopy Sciences, Hatfield, PA). Z-stack maximum projection images were obtained using LASX acquisition software linked to a Leica DMi8 TCS SP8 confocal microscope (Wetzlar, Germany) using a 20X oil immersion objective. Images were subsequently tiled and stitched using LASX or Adobe Photoshop Software (Adobe Systems Inc., San Jose, CA) to generate representative photomicrographs.
Quantification of tdTomato+/NeuN+ aDGCs and c-Fos+ nuclei
The number of tdTomato+/NeuN+ co-labelled aDGCs or c-Fos+ nuclei were quantified using the Optical Fractionator probe in StereoInvestigator software (MicroBrightfield Biosciences, Williston, VT) linked to an Olympus IX-51 DSU spinning disk confocal microscope using a 40X objective as previously described . For comparing tdTomato+/NeuN+ aDGCs across groups, the number of co-labeled aDGCs within the dentate gyrus of the dorsal hippocampus were estimated across two coronal sections per mouse. Regions of interest (ROI) were manually outlined using a 10X objective and followed the outer limits of the granule cell layer and 1 cell thickness into the hilus. Two sections spaced 480μm apart between –1.28 and –2.12 mm relative to bregma were quantified per mouse using an exhaustive counting scheme with 150×150μm grid size. The numbers of c-Fos positive nuclei within the suprapyramidal blade of the dorsal dentate gyrus were similarly estimated utilizing three coronal sections (between –1.28 and –2.12 to bregma) spaced 240μm apart and an identical exhaustive counting scheme. The optical disector height was set at 15μm with 2μm top and bottom guard zones.
Dendritic morphological analysis
For dendritic morphological analysis, PAE and SAC NestinCreERT2:tdTomato mice received a single dose of tamoxifen (180 mg/kg, intraperitoneal) at 47–51 days of age and were maintained in EE housing conditions until sacrifice at 6 weeks post-tamoxifen (∼3.5 months of age at sacrifice). Mice were sacrificed by sodium pentobarbital followed by transcardial perfusion with 4% paraformaldehyde, with post-fixation and tissue cryoprotection as described above. Brains were sectioned in the coronal plane at 60–100μm thickness, mounted on glass slides and coverslipped with Fluoromount G as described above. Z-stack images were obtained using the Leica DMi8 TCS SP8 confocal microscope and LASX acquisition software. A 20× oil immersion objective (1μm optical sections) was used to capture the dendritic tree and a 63× oil immersion objective (zoom 5, and z-step size of 0.15μm) was used to capture spine images. Z stacks of dendrites were subsequently processed into maximum projection 2D images for further analysis. Spine images were processed using Huygens Deconvolution (Scientific Volume Imaging, Hilversum, Netherlands) prior to 3D reconstruction using ImarisTM image analysis software (Bitplane, Concord, MA).
Dendrites from individual tdTomato+ aDGCs were traced using 2D Neurolucida software (MBF Bioscience, Williston, VT) and analyzed for branching complexity (Sholl analysis), total dendritic length and cell body area using Neurolucida Explorer. Dendritic protrusions (spines) were imaged from segments 20–75μm in length (40μm average) located within the middle 1/3 of the dendritic tree of tdTomato+ aDGCs located within the suprapyramidal blade of the dentate gyrus. Dendritic segments were digitally reconstructed in 3D utilizing Imaris Filament Tracer and Spine Detection Software (Bitplane, Concord, MA), and subjected to automated spine quantification and classification using Imaris Classify Spines Xtension. Dendritic protrusions were binned into the following categories using default measurement rules that are based on morphological maturity (Toni et al., 2007; Berry and Navadi, 2017): Stubby spines (<1μm in length), long thin spines (protrusion > 3μm in length with head width > neck width), mushroom spines (protrusion length < 3μm and a head width > 2× neck width) and filopodia (remaining dendritic protrusions). Two to five tdTomato+ aDGCs were sampled and averaged for each mouse for Sholl analysis (n/group = mouse number/group), and 3 dendritic sections were sampled per mouse for spine analysis (n/group = mouse number/group), with 4–6 mice per group across 2–6 distinct litters as indicated in figure legends.
This study was restricted to male offspring for consistency due to limited availability of mice for some experiments. Future work will focus on characterization of female mice, given that prior research has demonstrated sex differences in vulnerability to alcohol toxicity [34, 35].
All data were analyzed using ANOVA with appropriate post-hoc analyses as indicated in text using GraphPad Prism 8.3.0 (La Jolla, CA). Data are expressed as means±SEM, with p-values < 0.05 considered significant.
Impaired EE-mediated improvement in discriminative learning in PAE mice is directly correlated with impaired neurogenesis
The A-B contextual-fear discrimination behavioral assay tests the cognitive ability of mice to learn to discriminate between two similar contexts [24, 36] and requires the activity of adult-generated DGCs (aDGCs) for optimal performance [30–32, 37–39]. Here, we tested whether impairment of EE-mediated neurogenesis in PAE mice is directly correlated with impaired performance on this behavioral task. Figures 1A and 1B depict the experimental timeline and 7 day behavioral testing protocol, respectively. Briefly, SAC and PAE NestinCreERT2:tdTomato male offspring received daily tamoxifen injections for five consecutive days at one week post-weaning to induce tdTomato reporter gene expression in hippocampal nestin+ stem/progenitor cells and their downstream progeny [21, 22, 28, 30, 31], and were subsequently placed into standard housing (SH) or enriched environment (EE) for 10 weeks. We then tested all mice on a neurogenesis-dependent version of the A-B context-fear discrimination learning task, where mice learn to discriminate the shock context (context A) from a similar but distinct non-shock context (context B). Discrimination was quantified using an automated scoring method based on freezing behavior as previously described [22, 30, 31]. We began testing all mice at 10 weeks post-tamoxifen, as prior work demonstrated that PAE-EE mice display ∼50% fewer aDGCs compared to SAC-EE mice at this time point [20–22].
Although all mice learned the discrimination task over time, there were significant differences across groups. Analysis of the discrimination ratios by three-way repeated measures ANOVA revealed significant discrimination learning across all groups over time (testing day F(6,250) = 325, p < 0.0001, Fig. 1C). Significant main effects of alcohol treatment [F(1,250) = 175, p < 0.0001] and housing [F(1,250) = 12.49, p = 0.0005)] were detected, with significant interactions of treatment x housing [F(1,250) = 98.73, p < 0.0001] and treatment x housing x day [F(6,250) = 18.38, p < 0.0001]. As shown in Fig. 1D, by testing day 7 SAC-EE mice displayed an approximate 2-fold increase in discrimination ability compared to their SAC-SH controls (p < 0.0001; Tukey’s post-hoc multiple comparison). In contrast, discrimination scores of PAE-EE mice were no different from PAE-SH mice at day 7. Figure 1E depicts average freezing times in context A and context B across all groups on testing day 7, demonstrating that the improved discrimination scores were due to reduced freezing time in the non-shock context, with SAC-EE mice showing greatest improvement compared to all other groups. These data demonstrate that EE-mediated improvement in a neurogenesis-dependent version of the A-B contextual fear discrimination task is impaired in PAE mice.
To assess the relationship between behavioral performance and adult hippocampal neurogenesis, we sacrificed all mice within one week of the final behavioral testing day (mice age ∼4 months) and quantified the number of tdTomato+ aDGCs within the dorsal dentate gyrus from 4 randomly selected mice per group. As shown in Fig. 2A, tdTomato+/NeuN+ aDGCs were robustly labeled across all groups, with an obvious increase in the number of aDGCs in SAC-EE mice. As shown in Fig. 2B, SAC-EE mice displayed a > 2-fold increase in the number of tdTomato+ aDGCs compared to SAC-SH controls (p = 0.02), whereas PAE-EE mice displayed no change in the number of tdTomato+ aDGCs compared to PAE-SH mice, consistent with previous reports [20–22]. Two-way ANOVA of tdTomato+ aDGC cell counts revealed main effects of alcohol treatment [F (1,11) = 5.83, p = 0.03)], housing [F(1,11) = 6.74, p = 0.02] and a significant treatment x housing interaction [F(1,11) = 8.07, p = 0.01]. Importantly, the number of aDGCs across individual mice was directly correlated with the level of performance on the neurogenesis-dependent A-B contextual fear-discrimination task at training day 7 (R2 = 0.9422), such that mice with the highest number of aDGCs displayed the highest discrimination scores and vice versa (Fig. 2C).
Increased c-Fos expression within the dentate gyrus granule cell layer of PAE-EE mice following exposure to novel environment
Recent work suggests that young aDGC contribute to pattern discrimination by facilitating sparse activation within the dentate granule cell layer via local feedback inhibition and/or via direct monosynaptic input onto mature DGCs [40, 41]. To determine whether impaired EE-mediated neurogenesis in PAE mice is associated with alterations in behavioral activation of pre-existing, mature DGCs, we compared expression of the immediate early gene, c-Fos, within the dentate granule cell layer of SAC-EE and PAE-EE mice following exposure to novel environment. For these experiments, we exposed SAC-EE and PAE-EE mice (both groups reared under EE for 8 weeks) to a novel open field environment for 30 minutes and sacrificed them 80 minutes after return to their home cage (Fig. 3A). Control mice were not exposed to the novel environment and remained undisturbed within their home cage until sacrifice. We then quantified the number of c-Fos+ nuclei within the superior blade of the dentate granule cell layer of all mice. As shown in Fig. 3C, c-Fos+ cells were sparsely distributed throughout the dentate granule cell layer in both groups exposed to novelty; however, PAE-EE mice displayed a > 2-fold increase in the mean number of c-Fos+ DGCs compared to SAC-EE mice (Fig. 3B). Two-way ANOVA revealed a significant alcohol treatment x novelty interaction [[F (1,21) = 10.03, p = 0.005] with a significant impact of novelty on c-Fos+ expression only in PAE-EE mice (p = 0.01, PAE-EE home cage vs. PAE-EE novelty, Tukey’s post-hoc analysis).
PAE impairs EE-mediated structural maturation of aDGCs
EE and spatial learning enhance dendritic complexity of aDGCs in mice [42–48]. Here, we examined the impact of PAE on EE-mediated dendritic branching complexity, using Sholl analysis and computer-assisted reconstruction of dendritic spines. For these studies, Nestin-CreERT2:tdTomato reporter mice received a single injection of tamoxifen at approximately 3 months of age to sparsely label aDGCs. Such sparse labeling facilitates dendritic tracing by minimizing dendritic tree overlap among labeled aDGCs. All mice were sacrificed at 6 weeks post-labeling (mice ∼4.5 months of age at sacrifice), dendritic trees were traced and their morphology assessed by computer assisted Sholl analysis. As shown in Fig. 4A, dendritic branching was highest in SAC-EE mice compared to all other groups. Three-way ANOVA of dendritic branching complexity revealed a significant effect of distance from cell soma [F(26, 216) = 53.90, p < 0.0001), alcohol treatment [F(1,216) = 12.27, p < 0.0006] and housing [F(1,162) = 9.40, p < 0.0025] on dendritic branching, with a significant alcohol treatment x housing interaction [(F(1,162) = 24.70), p < 0.0001], but no change in total dendritic length (Fig. 4D). Two-way ANOVA comparison of total intersections across groups revealed a significant alcohol treatment x housing interaction [F(1,14) = 6.05, p = 0.027] with SAC-EE>PAE-EE (p = 0.016, Sidak’s multiple comparison). Representative traces of aDGC dendrites in 2D are shown in Fig. 1B. The increased dendritic complexity in SAC-EE mice was also accompanied by a significant increase in cell body size compared to SAC-SH controls, which was not observed in PAE-EE mice (Fig. 4C).
To determine whether impairment of EE-mediated dendritic branching in aDGCs from PAE mice was associated with alterations in dendritic spine density or morphology, we sampled 20–75μm dendritic segments (average 40μm) located within the middle third of the dendritic tree from randomly selected SAC-EE and PAE-EE tdTomato+ aDGCs. We digitally reconstructed dendritic segments and subjected the reconstructions to automated quantification and classification of dendritic protrusions based on pre-determined morphological criteria as outlined in Methods and depicted in Fig. 5D. Total spine density of 6 week old aDGCs did not differ significantly between SAC-EE and PAE-EE mice (Fig. 5A). However, classification of spine type by morphology revealed 50% fewer filopodia-like spines in 6 week old aDGCs from PAE-EE compared to SAC-EE mice (Fig. 5B). When calculated as percentage of total spines (Fig. 5C), the reduced number of filopodial protrusions was accompanied by an increase in percentage of mushroom spines in PAE-EE (4.14% filopodial, 10.17% mushroom) compared to SAC-EE mice (8.44% filopodial, 8.77% mushroom); however, this increased percentage of mushroom spines did not reach statistical significance (Chi-square statistical analysis), and there were also no significant differences in the distribution of other spine types.
We found that PAE impairs EE-mediated improvement of discrimination learning as assessed using the A-B contextual fear discrimination task, and that impaired learning is directly correlated with impaired EE-mediated neurogenesis across individual mice. Previous work has demonstrated that immature aDGCs uniquely impact hippocampal network activity and are critical for pattern discrimination learning . In the A-B contextual fear discrimination task, the mouse acquires a discrimination over training days, such that fear responses gradually become less in the non-shock compared to the shock context. Suppression of adult neurogenesis, optogenetic silencing of adult generated DGCs, or interference with their plasticity increases generalization between fear and neutral contexts and impairs discrimination learning of this task over time . The ability to discriminate similar episodic memories with overlapping features, such as required for the A-B contextual fear discrimination and other pattern separation tasks depends upon functional adult hippocampal neurogenesis [11, 14, 32, 39]. Sahay et al. originally demonstrated that enhancing neurogenesis using a gain of function genetic approach was sufficient to improve learning on the A-B context fear discrimination task  and we previously demonstrated that genetic silencing of aDGCs without impairing their survival markedly impaired learning in this behavioral paradigm . The current studies extend prior work demonstrating impaired A-B context discrimination learning in PAE-EE vs. SAC-EE mice , by also comparing learning across SH conditions and by demonstrating a direct correlation between the rate of neurogenesis and behavioral performance on an individual mouse basis. Our observation that PAE-SH mice display similar levels of discrimination learning compared to SAC-SH controls by testing day 7 suggests that, at least in this behavioral test of discrimination learning, non-neurogenic mechanisms related to general disruption(s) of hippocampal function in PAE do not mask neurogenesis-dependent performance.
The mechanisms by which aDGCs promote pattern discrimination learning are not entirely understood, but are thought to involve engagement of inhibitory circuits that facilitate sparse encoding of contextual and spatial information by the dentate granule cell layer [10, 40, 41, 50]. Young aDGCs are less constrained by GABAergic inhibitory input [51–56], are more easily recruited by cortical inputs [57, 58] and exert greater recruitment of GABAergic inhibitory interneurons . Impaired neurogenesis is thereby thought to result in reduced inhibition of mature dentate granule cells, which in turn leads to overlapping patterns of neuronal activation in response to similar but non-identical stimuli [10, 32, 40, 41, 50]. Inhibition of mature, pre-existing DGCs by young aDGCs not only occurs via indirect disynaptic feedback that involves favored recruitment of GABAergic inhibitory interneurons  but can also occur via direct monosynaptic innervation of mature DGCs . In the latter case, presynaptic glutamate released by aDGCs stimulates extrasynaptic group II metabotropic glutamate receptors (mGluRII) coupled to G-protein inwardly rectifying potassium channels (GIRKs) expressed on mature DGCs . This occurs primarily in response to activation of aDGCs by the lateral entorhinal cortex(LEC) /lateral performant path (LPP), which is particularly important in transmitting novel information [41, 60, 61]. LPP inputs to the dentate gyrus are most pronounced in the suprapyramidal blade [41, 62] and aDGCs receive greater innervation from the LEC than from medial entorhinal cortex [63, 64]. Consistent with a role for aDGCs in these inhibitory processes, we observed an ∼ 2-fold increase in the number of novelty-activated dentate granule cells within the suprapyramidal blade in PAE-EE compared to SAC-EE mice based on expression of the immediate early gene, c-Fos, a surrogate marker for neuronal activation at the population level. This result was anticipated, given that there are ∼50% fewer aDGCs in PAE-EE compared to SAC-EE mice. This level of disinhibition of mature dentate granule cells is also comparable to that reported in other studies in which adult neurogenesis has been arrested [40, 41]. It should be noted that recent studies have identified a rare and spatially localized subtype of developmentally-generated DGC that dominates granule cell recruitment preferentially within the suprapyramidal blade in response to a range of novelty experiences, but whether these are regulated by young aDGCs has not been investigated .
In contrast to PAE-EE mice, SAC-EE mice displayed no increase in c-Fos activation within the dentate gyrus in response to novelty. This is also consistent with prior studies demonstrating that normal mice exposed to EE for prolonged periods habituate faster to novelty compared to SH mice [66, 67]. Stimulation of adult-born granule cells in vivo lowers the number of c-Fos compared with controls during novel arena exploration . Taken together, these observations indicate that PAE mice do not appropriately adapt to enriched environment, and strongly suggest that impaired EE-mediated neurogenesis has important consequences for behavioral activation of inhibitory hippocampal circuits thought to mediate both novelty and pattern discrimination learning.
In addition to asking how impaired EE-mediated neurogenesis in PAE mice impacts circuit activation and behavioral output, it is also important to understand whether surviving aDGCs in PAE-EE mice display compensatory mechanisms in an attempt to meet increased hippocampal-engaged cognitive demand with fewer aDGCs. We previously demonstrated that aDGCs from PAE-EE mice display a marked increase in the frequency of spontaneous excitatory postsynaptic currents compared to aDGCs in SAC-EE mice . This suggests a compensatory increase in monosynaptic excitatory afferent input, perhaps in an attempt to meet increased cognitive demand with 50% fewer aDGCs in PAE-EE compared to SAC-EE mice. Prior work has demonstrated that engagement of hippocampal activity and conditions of EE promote dendritic complexity and increased monosynaptic afferent input [42–44, 46, 47]. Surprisingly, we observed a significant impairment of EE-mediated dendritic branching of aDGCs in PAE mice and also an impairment of EE-mediated neuronal maturation as assessed by cell body size, indicating reduced EE-mediated structural plasticity of aDGCs within PAE mice.
Although spine densities were similar in SAC-EE and PAE-EE mice, filopodial protrusions were decreased by approximately 50% in PAE-EE mice, and this was accompanied by an increase in the density of mature mushroom spines that did not reach statistical significance. Filopodia are actin-rich protrusions that emerge from dendrites during neuronal maturation to form contacts with nearby axons . Upon synaptic contact, filopodia transform into thin or mushroom spines with functional synapses or may be pruned if they do not receive inputs . Previous work has demonstrated that both EE and direct high frequency stimulation of performant path inputs to aDGCs increase spine size without changing total spine density [42, 71]. Since our studies were powered to detect >30% differences in spine type, it is difficult to determine whether the decreased number of filopodial protrusions in PAE-EE mice might represent filopodia pruning due to reduced synaptic input vs. ongoing transition of filopodial to more mature spine types that did not reach statistical significance at this time point. This would be most parsimonious with our prior electrophysiological findings of increased spontaneous excitatory synaptic activity in aDGCs from PAE-EE vs. SAC-EE mice, since mushroom spines contain the highest number of postsynaptic glutamatergic receptors , thereby providing a substrate for increased afferent excitatory synaptic contact. On the other hand, our findings of impaired EE-mediated dendritic branching and cell body size together with fewer filopodia also supports the former possibility (i.e., reduced synaptic input). It is important to note that in the current study, we assessed dendritic branching and spines in 6 week old aDGCs that experienced 6 weeks of EE, whereas the electrophysiological studies were performed in 8 week old aDGCs exposed to 8 weeks of EE. Thus these different maturational time points could also account for the disparity. An additional caveat includes the region of dendrite analysis, which was restricted to the middle third of the dendritic tree in the current study. This may be relevant, since prior studies have demonstrated heterotopic/homeostatic remodeling of spines along the dendritic tree of aDGCs, depending on source of evoked excitatory activity . Nevertheless, our observations of a 50% reduction of filopodial protrusions in PAE-EE mice warrants further investigation to determine the extent, time course and functional consequences of changes in spine morphologies in PAE-EE vs. SAC-EE mice.
We have previously demonstrated that impaired EE-mediated neurogenesis in PAE mice is largely due to impaired integration of newly generated post-mitotic neurons, and not impaired progenitor proliferation, reduced pool of early progenitors or decreased exploratory activity within the complex enriched environment [20–22, 68]. Potential mechanisms for impaired EE-mediated adult neurogenesis may include altered electrophysiological function of the existing hippocampal circuitry and/or inability of newly generated aDGCs to respond appropriately to activity-dependent integration. Activity-dependent synaptic integration into a functional hippocampal circuit is essential for long-term survival of early post-mitotic aDGCs . Neurogenesis is tightly linked to excitation [73, 74], and EE-mediated neurogenesis requires activation of existing DGCs . Newborn aDGCs require activity-dependent synaptic input for survival during a critical period in their maturation when they display heightened plasticity and lowered threshold for long-term potentiation (LTP) , dependent upon appropriate NMDA receptor subunit expression . EE also exerts profound effects on afferent connectivity, including a topographical redistribution of monosynaptic afferent input onto newborn aDGCs that may be important for EE-mediated survival . Thus, disruption of existing circuitry or perturbation of NMDA receptor subunit expression in newly-generated aDGCs in PAE mice could impair activity-dependent survival. PAE is known to result in decreased dentate LTP [76–79] and decreased expression of NMDA receptor subunits . We previously demonstrated impairment of EE-mediated dendritic branching in pre-existing mature DGCs in PAE mice that was associated with blunting of an EE-mediated shift in the excitatory/inhibitory balance of spontaneous synaptic input , further indicating that the inappropriate response of the existing circuitry to EE may underlie impaired EE-mediated neurogenesis.
Prior studies utilizing various gestational exposure paradigms have demonstrated that higher doses of alcohol administered throughout gestation or during the early postnatal period in rodents can cause a reduction in baseline neurogenesis, which can be restored back to baseline by EE or exercise [81–83]. However, our studies focus on a moderate gestational alcohol exposure paradigm, in which baseline neurogenesis is unaffected under standard housing conditions, but mice are unable to elicit a neurogenic response to EE above baseline. This effect is dependent upon timing of gestational exposure and does not occur when ethanol exposure is restricted to the first 2 weeks of postnatal life . The relevance of this work for clinical FASD is underscored by mounting evidence for continuation of hippocampal neurogenesis throughout life in humans [84–87] [but see ], and the role of neurogenesis in hippocampal-dependent cognitive and behavioral deficits that are also disrupted in FASD [16–19, 89]. Reductions in hippocampal volume and impaired temporal lobe network function that correlate with hippocampal-related behavioral deficits in FASD are also consistent with the possibility of impaired neurogenic function [7, 90–93].
One limitation of the current study is that only males were included. While a complete analysis of sex-dependent effects was beyond the scope of the current study, we previously found no significant sex-dependent effects of PAE on EE-mediated neurogenesis, although the overall level of EE-mediated neurogenesis in SAC-EE mice was slightly greater in females than males . On the other hand, there is evidence that prenatal alcohol can exert sex-dependent effects on structural and functional outcomes [94, 95], and sex-dependent effects on adult neurogenesis after learning have also been reported . Thus, it will be important in future studies to determine whether the behavioral and structural correlates of impaired EE-mediated neurogenesis described here also occur in female PAE mice.
In conclusion, our studies support behavioral relevance for impaired EE-mediated neurogenesis in PAE mice, and provide an additional basis for testing therapeutic strategies that might restore EE-mediated plasticity in our preclinical FASD mouse model of moderate gestation alcohol exposure. The implication is that restoring the neurogenic response to cognitive demand may be required for optimal responsiveness to behavioral training/interventions that target hippocampal-associated behaviors in clinical FASD .
This research was directly supported by the following grants: NIH-NIAAA P50-AA022534; NIH-NIAAA F31AA027127; NIH-NIAAA 1R01AA027462-01A1; NIH-NIGMS 1P20GM109089.
CONFLICT OF INTEREST
The authors have no conflicts of interest to report.
Images were acquired using the UNM-HSC Cancer Center Fluorescence Microscopy & Cell Imaging Shared Resource that is supported by a UNM Comprehensive Center Support Grant P30CA118100.
Greenmyer JR , Popova S , Klug MG , Burd L . Fetal alcohol spectrum disorder: a systematic review of the cost of and savings from prevention in the United States and Canada. Addiction. 2020;115(3):409–17.
May PA , Baete A , Russo J , Elliott AJ , Blankenship J , Kalberg WO , et al. Prevalence and characteristics of fetal alcohol spectrum disorders. Pediatrics. 2014;134(5):855–66.
Tan CH , Denny CH , Cheal NE , Sniezek JE , Kanny D . Alcohol use and binge drinking among women of childbearing age - United States, 2011–2013. MMWR Morbidity and mortality weekly report. 2015;64(37):1042–6.
Kodituwakku PW , Kodituwakku EL . From research to practice: an integrative framework for the development of interventions for children with fetal alcohol spectrum disorders. Neuropsychology Review. 2011;21(2):204–23.
Petrelli B , Weinberg J , Hicks GG . Effects of Prenatal Alcohol Exposure (PAE): Insights into FASD using PAE Mouse Models. Biochem Cell Biol. 2018.
Autti-Ramo I , Autti T , Korkman M , Kettunen S , Salonen O , Valanne L . MRI findings in children with school problems who had been exposed prenatally to alcohol. Dev Med Child Neurol. 2002;44(2):98–106.
Berman RF , Hannigan JH . Effects of prenatal alcohol exposure on the hippocampus: spatial behavior, electrophysiology, and neuroanatomy. Hippocampus. 2000;10(1):94–110.
Nardelli A , Lebel C , Rasmussen C , Andrew G , Beaulieu C . Extensive deep gray matter volume reductions in children and adolescents with fetal alcohol spectrum disorders. Alcohol Clin Exp Res. 2011;35(8):1404–17.
Willoughby KA , Sheard ED , Nash K , Rovet J . Effects of prenatal alcohol exposure on hippocampal volume, verbal learning, and verbal and spatial recall in late childhood. J Int Neuropsychol Soc. 2008;14(6):1022–33.
Anacker C , Hen R . Adult hippocampal neurogenesis and cognitive flexibility - linking memory and mood. Nat Rev Neurosci. 2017;18(6):335–46.
Clelland CD , Choi M , Romberg C , Clemenson GD Jr , Fragniere A , Tyers P et al. A functional role for adult hippocampal neurogenesis in spatial pattern separation. Science. 2009;325(5937):210–3.
Danielson NB , Kaifosh P , Zaremba JD , Lovett-Barron M , Tsai J , Denny CA , et al. Distinct Contribution of Adult-Born Hippocampal Granule Cells to Context Encoding. Neuron. 2016;90(1):101–12.
Denny CA , Burghardt NS , Schachter DM , Hen R , Drew MR . 4- to 6-week-old adult-born hippocampal neurons influence novelty-evoked exploration and contextual fear conditioning. Hippocampus. 2012;22(5):1188–201.
Nakashiba T , Cushman JD , Pelkey KA , Renaudineau S , Buhl DL , McHugh TJ , et al. Young dentate granule cells mediate pattern separation, whereas old granule cells facilitate pattern completion. Cell. 2012;149(1):188–201.
Swan AA , Clutton JE , Chary PK , Cook SG , Liu GG , Drew MR . Characterization of the role of adult neurogenesis in touch-screen discrimination learning. Hippocampus. 2014;24(12):1581–91.
Hellemans KG , Sliwowska JH , Verma P , Weinberg J . Prenatal alcohol exposure: fetal programming and later life vulnerability to stress, depression and anxiety disorders. Neurosci Biobehav Rev. 2010;34(6):791–807.
O’Connor MJ , Paley B . Psychiatric conditions associated with prenatal alcohol exposure. Dev Disabil Res Rev. 2009;15(3):225–34.
Pei J , Denys K , Hughes J , Rasmussen C . Mental health issues in fetal alcohol spectrum disorder. J Ment Health. 2011;20(5):438–48.
Streissguth AP , O’Malley K . Neuropsychiatric implications and long-term consequences of fetal alcohol spectrum disorders. Seminars in Clinical Neuropsychiatry. 2000;5(3):177–90.
Choi IY , Allan AM , Cunningham LA . Moderate fetal alcohol exposure impairs the neurogenic response to an enriched environment in adult mice. Alcohol Clin Exp Res. 2005;29(11):2053–62.
Cunningham LA , Newville J , Li L , Tapia P , Allan AM , Valenzuela CF . Prenatal Alcohol Exposure Leads to Enhanced Serine 9 Phosphorylation of Glycogen Synthase Kinase-3beta (GSK-3beta) in the Hippocampal Dentate Gyrus of Adult Mouse. Alcohol Clin Exp Res. 2017;41(11):1907–16.
Kajimoto K , Allan A , Cunningham LA . Fate analysis of adult hippocampal progenitors in a murine model of fetal alcohol spectrum disorder (FASD). PLoS ONE. 2013;8(9):e73788.
Clemenson GD , Henningfield CM , Stark CEL . Improving Hippocampal Memory Through the Experience of a Rich Minecraft Environment. Front Behav Neurosci. 2019;13:57.
Clemenson GD , Lee SW , Deng W , Barrera VR , Iwamoto KS , Fanselow MS , et al. Enrichment rescues contextual discrimination deficit associated with immediate shock. Hippocampus. 2015;25(3):385–92.
Ethen MK , Ramadhani TA , Scheuerle AE , Canfield MA , Wyszynski DF , Druschel CM , et al. Alcohol consumption by women before and during pregnancy. Maternal and Child Health Journal. 2009;13(2):274–85.
Lagace DC , Whitman MC , Noonan MA , Ables JL , DeCarolis NA , Arguello AA , et al. Dynamic contribution of nestin-expressing stem cells to adult neurogenesis. J Neurosci. 2007;27(46):12623–9.
Madisen L , Zwingman TA , Sunkin SM , Oh SW , Zariwala HA , Gu H , et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nature Neuroscience. 2010;13(1):133–40.
Gustus K , Lozano E , Newville J , Li L , Valenzuela CF , Cunningham LA . Resistance of Postnatal Hippocampal Neurogenesis to Alcohol Toxicity in a Third Trimester-Equivalent Mouse Model of Gestational Alcohol Exposure. Alcohol Clin Exp Res. 2019.
Brady ML , Allan AM , Caldwell KK . A limited access mouse model of prenatal alcohol exposure that produces long-lasting deficits in hippocampal-dependent learning and memory. Alcoholism, Clinical and Experimental Research. 2012;36(3):457–66.
Carrica L , Li L , Newville J , Kenton J , Gustus K , Brigman J , Cunningham LA . Genetic inactivation of hypoxia inducible factor 1-alpha (HIF-1alpha) in adult hippocampal progenitors impairs neurogenesis and pattern discrimination learning. Neurobiology of Learning and Memory. 2018;157:79–85.
Gustus KC , Li L , Chander P , Weick JP , Wilson MC , Cunningham LA . Genetic inactivation of synaptosomal-associated protein 25 (SNAP-25) in adult hippocampal neural progenitors impairs pattern discrimination learning but not survival or structural maturation of newborn dentate granule cells. Hippocampus. 2018;28(10):735–44.
Sahay A , Scobie KN , Hill AS , O’Carroll CM , Kheirbek MA , Burghardt NS , et al. Increasing adult hippocampal neurogenesis is sufficient to improve pattern separation. Nature. 2011;472(7344):466–70.
Huckleberry KA , Shue F , Copeland T , Chitwood RA , Yin W , Drew MR . Dorsal and ventral hippocampal adult-born neurons contribute to context fear memory. Neuropsychopharmacology. 2018.
Goodlett CR , Peterson SD . Sex differences in vulnerability to developmental spatial learning deficits induced by limited binge alcohol exposure in neonatal rats. Neurobiol Learn Mem. 1995;64(3):265–75.
Sharrett-Field L , Butler TR , Reynolds AR , Berry JN , Prendergast MA . Sex differences in neuroadaptation to alcohol and withdrawal neurotoxicity. Pflugers Arch. 2013;465(5):643–54.
McHugh TJ , Jones MW , Quinn JJ , Balthasar N , Coppari R , Elmquist JK , et al. Dentate gyrus NMDA receptors mediate rapid pattern separation in the hippocampal network. Science. 2007;317(5834):94–9.
Kheirbek MA , Klemenhagen KC , Sahay A , Hen R . Neurogenesis and generalization: a new approach to stratify and treat anxiety disorders. Nature Neuroscience. 2012;15(12):1613–20.
Kheirbek MA , Tannenholz L , Hen R . NR2B-dependent plasticity of adult-born granule cells is necessary for context discrimination. J Neurosci. 2012;32(25):8696–702.
Tronel S , Belnoue L , Grosjean N , Revest JM , Piazza PV , Koehl M , et al. Adult-born neurons are necessary for extended contextual discrimination. Hippocampus. 2012;22(2):292–8.
Drew LJ , Kheirbek MA , Luna VM , Denny CA , Cloidt MA , Wu MV , et al. Activation of local inhibitory circuits in the dentate gyrus by adult-born neurons. Hippocampus. 2016;26(6):763–78.
Luna VM , Anacker C , Burghardt NS , Khandaker H , Andreu V , Millette A , et al. Adult-born hippocampal neurons bidirectionally modulate entorhinal inputs into the dentate gyrus. Science. 2019;364(6440):578–83.
Bergami M , Masserdotti G , Temprana SG , Motori E , Eriksson TM , Gobel J , et al. A critical period for experience-dependent remodeling of adult-born neuron connectivity. Neuron. 2015;85(4):710–7.
Lemaire V , Tronel S , Montaron MF , Fabre A , Dugast E , Abrous DN . Long-lasting plasticity of hippocampal adult-born neurons. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience. 2012;32(9):3101–8.
Pallas-Bazarra N , Jurado-Arjona J , Navarrete M , Esteban JA , Hernandez F , Avila J , et al. Novel function of Tau in regulating the effects of external stimuli on adult hippocampal neurogenesis. EMBO J. 2016;35(13):1417–36.
Trinchero MF , Buttner KA , Sulkes Cuevas JN , Temprana SG , Fontanet PA , Monzon-Salinas MC , et al. High Plasticity of New Granule Cells in the Aging Hippocampus. Cell Rep. 2017;21(5):1129–39.
Trinchero MF , Herrero M , Monzon-Salinas MC , Schinder AF . Experience-Dependent Structural Plasticity of Adult-Born Neurons in the Aging Hippocampus. Front Neurosci. 2019;13:739.
Tronel S , Fabre A , Charrier V , Oliet SH , Gage FH , Abrous DN . Spatial learning sculpts the dendritic arbor of adult-born hippocampal neurons. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(17):7963–8.
Zhao C , Jou J , Wolff LJ , Sun H , Gage FH . Spine morphogenesis in newborn granule cells is differentially regulated in the outer and middle molecular layers. The Journal of Comparative Neurology. 2015;523(10):1588.
Drew MR , Huckleberry KA . Modulation of Aversive Memory by Adult Hippocampal Neurogenesis. Neurotherapeutics. 2017;14(3):646–61.
Deng W , Mayford M , Gage FH . Selection of distinct populations of dentate granule cells in response to inputs as a mechanism for pattern separation in mice. Elife. 2013;2:e00312.
Chancey JH , Adlaf EW , Sapp MC , Pugh PC , Wadiche JI , Overstreet-Wadiche LS . GABA depolarization is required for experience-dependent synapse unsilencing in adult-born neurons. J Neurosci. 2013;33(15):6614–22.
Ge S , Yang CH , Hsu KS , Ming GL , Song H . A critical period for enhanced synaptic plasticity in newly generated neurons of the adult brain. Neuron. 2007;54(4):559–66.
Gu Y , Arruda-Carvalho M , Wang J , Janoschka SR , Josselyn SA , Frankland PW , et al. Optical controlling reveals time-dependent roles for adult-born dentate granule cells. Nature Neuroscience. 2012;15(12):1700–6.
Marin-Burgin A , Mongiat LA , Pardi MB , Schinder AF . Unique processing during a period of high excitation/inhibition balance in adult-born neurons. Science. 2012;335(6073):1238–42.
Schmidt-Hieber C , Jonas P , Bischofberger J . Enhanced synaptic plasticity in newly generated granule cells of the adult hippocampus. Nature. 2004;429(6988):184–7.
Snyder JS , Cameron HA . Could adult hippocampal neurogenesis be relevant for human behavior? Behavioural Brain Research. 2012;227(2):384–90.
Dieni CV , Nietz AK , Panichi R , Wadiche JI , Overstreet-Wadiche L . Distinct determinants of sparse activation during granule cell maturation. J Neurosci. 2013;33(49):19131–42.
Mongiat LA , Esposito MS , Lombardi G , Schinder AF . Reliable activation of immature neurons in the adult hippocampus. PLoS One. 2009;4(4):e5320.
Alvarez DD , Giacomini D , Yang SM , Trinchero MF , Temprana SG , Büttner KA , et al. A disynaptic feedback network activated by experience promotes the integration of new granule cells. Science. 2016;354(6311):459–65.
Jessberger S , Clark RE , Broadbent NJ , Clemenson GD Jr , Consiglio A , Lie DC et al. Dentate gyrus-specific knockdown of adult neurogenesis impairs spatial and object recognition memory in adult rats. Learn Mem. 2009;16(2):147–54.
Suarez-Pereira I , Carrion AM . Updating stored memory requires adult hippocampal neurogenesis. Sci Rep. 2015;5:13993.
Witter MP . The perforant path: projections from the entorhinal cortex to the dentate gyrus. Prog Brain Res. 2007;163:43–61.
Vivar C , Potter MC , Choi J , Lee JY , Stringer TP , Callaway EM , et al. Monosynaptic inputs to new neurons in the dentate gyrus. Nature Communications. 2012;3:1107.
Woods NI , Vaaga CE , Chatzi C , Adelson JD , Collie MF , Perederiy JV , et al. Preferential Targeting of Lateral Entorhinal Inputs onto Newly Integrated Granule Cells. J Neurosci. 2018;38(26):5843–53.
Erwin SR , Sun W , Copeland M , Lindo S , Spruston N , Cembrowski MS . A Sparse, Spatially Biased Subtype of Mature Granule Cell Dominates Recruitment in Hippocampal-Associated Behaviors. Cell Rep. 2020;31(4):107551.
van de Weerd HA . Evaluation of environmental enrichment for laboratory mice. Vet Q. 1997;19(sup1):59.
van de Weerd HA , Baumans V , Koolhaas JM , van Zutphen LF . Strain specific behavioural response to environmental enrichment in the mouse. J Exp Anim Sci. 1994;36(4-5):117–27.
Kajimoto K , Valenzuela CF , Allan AM , Ge S , Gu Y , Cunningham LA . Prenatal alcohol exposure alters synaptic activity of adult hippocampal dentate granule cells under conditions of enriched environment. Hippocampus. 2016;26(8):1078–87.
Kasai H , Matsuzaki M , Noguchi J , Yasumatsu N , Nakahara H . Structure-stability-function relationships of dendritic spines. Trends Neurosci. 2003;26(7):360–8.
Chidambaram SB , Rathipriya AG , Bolla SR , Bhat A , Ray B , Mahalakshmi AM , et al. Dendritic spines: Revisiting the physiological role. Prog Neuropsychopharmacol Biol Psychiatry. 2019;92:161–93.
Jungenitz T , Beining M , Radic T , Deller T , Cuntz H , Jedlicka P , et al. Structural homo- and heterosynaptic plasticity in mature and adult newborn rat hippocampal granule cells. Proc Natl Acad Sci U S A. 2018;115(20):E4670–E9.
Bergami M , Berninger B . A fight for survival: the challenges faced by a newborn neuron integrating in the adult hippocampus. Developmental Neurobiology. 2012;72(7):1016–31.
Deisseroth K , Singla S , Toda H , Monje M , Palmer TD , Malenka RC . Excitation-neurogenesis coupling in adult neural stem/progenitor cells. Neuron. 2004;42(4):535–52.
Song J , Zhong C , Bonaguidi MA , Sun GJ , Hsu D , Gu Y , et al. Neuronal circuitry mechanism regulating adult quiescent neural stem-cell fate decision. Nature. 2012;489(7414):150–4.
Kirschen GW , Shen J , Tian M , Schroeder B , Wang J , Man G , et al. Active Dentate Granule Cells Encode Experience to Promote the Addition of Adult-Born Hippocampal Neurons. J Neurosci. 2017;37(18):4661–78.
Brady ML , Diaz MR , Iuso A , Everett JC , Valenzuela CF , Caldwell KK . Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience. 2013;33(3):1062–7.
Helfer JL , White ER , Christie BR . Enhanced deficits in long-term potentiation in the adult dentate gyrus with 2nd trimester ethanol consumption. PloS One. 2012;7(12):e51344.
Sutherland RJ , McDonald RJ , Savage DD . Prenatal exposure to moderate levels of ethanol can have long-lasting effects on hippocampal synaptic plasticity in adult offspring. Hippocampus. 1997;7(2):232–8.
Varaschin RK , Akers KG , Rosenberg MJ , Hamilton DA , Savage DD . Effects of the cognition-enhancing agent ABT-239 on fetal ethanol-induced deficits in dentate gyrus synaptic plasticity. The Journal of Pharmacology and Experimental Therapeutics. 2010;334(1):191–8.
Samudio-Ruiz SL , Allan AM , Sheema S , Caldwell KK . Hippocampal N-methyl-D-aspartate receptor subunit expression profiles in a mouse model of prenatal alcohol exposure. Alcoholism, Clinical and Experimental Research. 2010;34(2):342–53.
Hamilton GF , Jablonski SA , Schiffino FL , St Cyr SA , Stanton ME , Klintsova AY . Exercise and environment as an intervention for neonatal alcohol effects on hippocampal adult neurogenesis and learning. Neuroscience. 2014;265:274–90.
Ieraci A , Herrera DG . Single alcohol exposure in early life damages hippocampal stem/progenitor cells and reduces adult neurogenesis. Neurobiology of Disease. 2007;26(3):597–605.
Redila VA , Olson AK , Swann SE , Mohades G , Webber AJ , Weinberg J , et al. Hippocampal cell proliferation is reduced following prenatal ethanol exposure but can be rescued with voluntary exercise. Hippocampus. 2006;16(3):305–11.
Boldrini M , Fulmore CA , Tartt AN , Simeon LR , Pavlova I , Poposka V , et al. Human Hippocampal Neurogenesis Persists throughout Aging. Cell Stem Cell. 2018;22(4):589–99 e5.
Eriksson PS , Perfilieva E , Björk-Eriksson T , Alborn A-M , Nordborg C , Peterson DA , et al. Neurogenesis in the adult human hippocampus. Nature Medicine. 1998;4(11).
Manganas LN , Zhang X , Li Y , Hazel RD , Smith SD , Wagshul ME , et al. Magnetic resonance spectroscopy identifies neural progenitor cells in the live human brain. Science. 2007;318(5852):980–5.
Spalding KL , Bergmann O , Alkass K , Bernard S , Salehpour M , Huttner HB , et al. Dynamics of hippocampal neurogenesis in adult humans. Cell. 2013;153(6):1219–27.
Sorrells SF , Paredes MF , Cebrian-Silla A , Sandoval K , Qi D , Kelley KW , et al. Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults. Nature. 2018.
Chokroborty-Hoque A , Alberry B , Singh SM . Exploring the complexity of intellectual disability in fetal alcohol spectrum disorders. Front Pediatr. 2014;2:90.
Benjamin LE , Keshet E . Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors: induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal. Proc Natl Acad Sci U S A. 1997;94(16):8761–6.
Dodge NC , Thomas KGF , Meintjes EM , Molteno CD , Jacobson JL , Jacobson SW . Reduced Hippocampal Volumes Partially Mediate Effects of Prenatal Alcohol Exposure on Spatial Navigation on a Virtual Water Maze Task in Children. Alcohol Clin Exp Res. 2020;44(4):844–55.
Riikonen R , Salonen I , Partanen K , Verho S . Brain perfusion SPECT and MRI in foetal alcohol syndrome. Developmental Medicine and Child Neurology. 1999;41(10):652–9.
Sowell ER , Lu LH , O’Hare ED , McCourt ST , Mattson SN , O’Connor MJ , et al. Functional magnetic resonance imaging of verbal learning in children with heavy prenatal alcohol exposure. Neuroreport. 2007;18(7):635–9.
Uban KA , Herting MM , Wozniak JR , Sowell ER , Cifasd . Sex differences in associations between white matter microstructure and gonadal hormones in children and adolescents with prenatal alcohol exposure. Psychoneuroendocrinology. 2017;83:111–21.
Weinberg J , Sliwowska JH , Lan N , Hellemans KG . Prenatal alcohol exposure: foetal programming, the hypothalamic-pituitary-adrenal axis and sex differences in outcome. J Neuroendocrinol. 2008;20(4):470–88.
Yagi S , Galea LAM . Sex differences in hippocampal cognition and neurogenesis. Neuropsychopharmacology. 2019;44(1):200–13.