Color standardization method and system for whole slide imaging based on spectral sensing

Issue title: 1st Congress of the International Academy of Digital Pathology Quebec City, Canada, August 3–5, 2011. Part II

Article type: Research Article

Authors: Tani, Shinsuke | Fukunaga, Yasuhiro | Shimizu, Saori | Fukunishi, Munenori | Ishii, Kensuke | Tamiya, Kosei

Affiliations: Olympus Corporation, Imaging Technology Department, Hachioji-shi, Tokyo, Japan | Olympus Corporation, Imager & Analog LSI Department, Hachioji, Tokyo, Japan | Olympus Corporation of Americas New Business Research & Development, Tokyo, Japan

Note: [] Corresponding author: Shinsuke Tani, Olympus Corporation, Imaging Technology Department, 2-3 Kuboyama-cho, Hachioji-shi, Tokyo 192-8512, Japan. Tel.: +81 42 691 8073; Fax: +81 42 691 7660; E-mail: [email protected]

Abstract: In the field of whole slide imaging, the imaging device or staining process cause color variations for each slide that affect the result of image analysis made by pathologist. In order to stabilize the analysis, we developed a color standardization method and system as described below. 1) Color standardization method based on RGB imaging and multi spectral sensing, which utilize less band (16 bands) than conventional method (60 bands). 2) High speed spectral sensing module. As a result, we confirmed the following effect. 1) We confirmed the performance improvement of nucleus detection by the color standardization. And we can conduct without training data set which is needed in conventional method. 2) We can get detection performance of H&E component equivalent to conventional method (60 bands). And measurement process is more than 255 times faster.

Keywords: Color standardization, color correction, dye amount, multi-spectral imaging, nucleus extraction

DOI: 10.3233/ACP-2011-0037

Journal: Analytical Cellular Pathology, vol. 35, no. 2, pp. 107-115, 2012