Journal of Berry Research - Volume Preprint, issue Preprint
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The main objective of the
Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems.
The objective will be achieved by focusing on four main areas of research and development:
1. From genetics to variety evaluation
2. Nursery production systems and plant quality control
3. Plant physiology, biochemistry and molecular biology, as well as cultural management
4. Health for the consumer: components and factors affecting berries' nutritional value
Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.
Journal of Berry Research will feature reviews, research articles, brief communications, position papers, letters and patent updates.
Abstract: BACKGROUND: Dielectric properties (DPs) are relevant parameters for microwave applications, such as sensors and heating for drying, mold control or disinfestation. DPs of berries are necessary in order to develop processes involving microwaves, reported with high potential for berries preservation. OBJECTIVE: To analyze the DPs in the microwave range and physicochemical properties of raspberry, strawberry and blackberry. METHODS: DPs were measured from 0.5 to 25 GHz at 20, 40 and 60°C using the open-ended coaxial probe method. Several physicochemical properties and biocompounds contents were determined. RESULTS: Dielectric constant increased with increasing temperature, and decreasing…with increasing frequency. Loss factor decreased with increasing temperature, and from 1.5 to 25 GHz, losses increased with increasing frequency. All berries had similar DP values, and microwaves had deeper penetration into strawberry (3.5 cm) than blackberry and raspberry samples (2.7 cm). CONCLUSIONS: Dielectric properties values are useful for further applications of microwaves for these berries, such as sensing, disinfestation, avoiding decay or drying.
Abstract: BACKGROUND: Visualization of ericoid mycorrhizal colonization using traditional methods relies on either fresh or KOH stored samples. Increasing interest in studying ericoid mycorrhization has highlighted a need for methods which can be used for preserved samples and are simple to implement with commonly available equipment. OBJECTIVE: The aim of this study was to improve on traditional techniques for staining ericoid mycorrhizal fungi and microscopically visualizing ericoid mycorrhizal roots which have been preserved. METHODS: Ericoid mycorrhizal roots were placed in KOH or frozen at – 20 °C for long-term storage. Traditional Trypan Blue staining methods were…modified to reduce damage to fine mycorrhizal hyphae and cortical cells. A high light-intensity dark-field microscopy technique was applied to clearly visualize stained mycorrhizae. The novel application was compared to other commonly used practices. RESULTS: Trypan Blue staining without KOH storage or clearing allowed for successful staining of ericoid mycorrhizal roots stored at – 20 °C. The application of high light-intensity dark-field microscopy provided high contrast visualization of mycorrhizal structures. CONCLUSIONS: The modified Trypan Blue staining method was effective on frozen root samples, with dark-field microscopy being particularly effective at visualizing dark colored roots. Advantages to this method are low cost and relatively fast application time. Therefore, this method is a realistic option for large scale analyses with many samples which require long-term preservation.
Keywords: Ericoid, mycorrhizae, dark-field microscopy, Trypan Blue staining, mycorrhizal colonization