Affiliations: [a] Department of Healthcare Engineering, Chonbuk National University, Korea | [b] Division of Biomedical Engineering, Chonbuk National University, Korea | [c] Research Center of Healthcare & Welfare Instrument for the Aged, Chonbuk National University, Korea | [d] LabGenomics Co., Ltd, Korea
Correspondence:
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Corresponding author: Mun-Ho Ryu, Division of Biomedical Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 561-756, Korea. E-mail:[email protected]
Abstract: There have been many studies to detect infectious
diseases with the molecular genetic method. This study presents an
automation process for a DNA extraction system based on microfluidics and
magnetic bead, which is part of a portable molecular genetic test system.
This DNA extraction system consists of a cartridge with chambers, syringes,
four linear stepper actuators, and a rotary stepper actuator. The actuators
provide a sequence of steps in the DNA extraction process, such as
transporting, mixing, and washing for the gene specimen, magnetic bead, and
reagent solutions. The proposed automation system consists of a PC-based
host application and an Arduino-based controller. The host application
compiles a G code sequence file and interfaces with the controller to
execute the compiled sequence. The controller executes stepper motor axis
motion, time delay, and input-output manipulation. It drives the stepper
motor with an open library, which provides a smooth linear acceleration
profile. The controller also provides a homing sequence to establish the
motor's reference position, and hard limit checking to prevent any
over-travelling. The proposed system was implemented and its functionality
was investigated, especially regarding positioning accuracy and velocity
profile.
Keywords: Molecular genetic test, DNA extraction, Arduino, automation
