Association of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer: A case-control study

Article type: Research Article

Authors: Huang, Baohua^a | Liu, Xiaoyan^b | Sun, Chengming^a | Wang, Lipeng^a | Yang, Liping^{a; *}

Affiliations: [a] Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China | [b] Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China

Correspondence: [*] Corresponding author: Liping Yang, Department of Laboratory, Yantai Yuhuangding Hospital, No. 20, Yuhuangding Eastern Road, Yantai 264000, Shandong, China. Tel.: +86 0535 6691999 83156; E-mail:[email protected]

Abstract: OBJECTIVE: To investigate the relationship of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer (CRC). METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used detect Bcl-2 gene polymorphisms (rs1800477A/G and rs1801018A/G) in 185 patients with CRC (case group) and 177 healthy subjects (control group). The relationships of Bcl-2 gene polymorphisms with clinicopathological features and prognosis of CRC patients were analyzed. RESULTS: The frequency of GG genotype and G allele of rs1800477A/G in the case group were significantly higher than those in the control group (both P < 0.05). The GG genotype of rs1800477A/G was associated with lymph node metastasis and Dukes' staging of CRC (both P < 0.05). Haplotype analysis demonstrated that the case group had decreased frequency of GA haplotype, but increased frequency of GG haplotype in comparisons to the control group (GA: P = 0.014; GG: P = 0.003). Kaplan-Meier survival analysis showed that the risk of death (within 5 years) in patients carrying GG genotype (rs1800477A/G) was 2.159 as much as that in patients carrying AA + GA genotype. Multivariate analyses showed that Dukes' staging and GG genotype of rs1800477A/G are risk factors for poor prognosis in CRC (Dukes' staging: P = 0.001; GG genotype: P = 0.034). CONCLUSION: Bcl-2 rs1800477A/G polymorphism may be related to the occurrence of CRC, and GG genotype could be a risk factor of poor prognosis in CRC.

Keywords: Colorectal cancer, Bcl-2, gene polymorphism, clinicopathological features, prognosis

DOI: 10.3233/CBM-160378

Journal: Cancer Biomarkers, vol. 18, no. 4, pp. 433-439, 2017