Affiliations: UMR 7561 CNRS – UHP Nancy 1, Physiopathologie, Pharmacologie et Ingénierie Articulaires, Université Nancy, Vandoeuvre-lès-Nancy, France | Chirurgie Orthopédique et Traumatologique, Hôpital Central, Nancy, France | Unité de Thérapie Cellulaire et Tissulaire, Nancy-Brabois, France
Note: [] Address for correspondence: Astrid Pinzano, PhD, UMR 7561 CNRS – Nancy Université, Physiopathologie, Pharmacologie et Ingénierie Articulaires, Faculté de Médecine, BP 184, Avenue de la Forêt de Haye, F54505 Vandoeuvre-lès-Nancy, France. Tel.: +33 383 683 950; Fax: +33 383 683 959; E-mail: [email protected].
Abstract: This study investigated the gene expression profile of human mesenchymal stem cells seeded in collagen sponge for 28 days in three different mediums: (1) basal medium as control containing ITS alone, (2) ITS+TGF-β1 alone or (3) ITS 1% supplemented sequentially by TGF-β1 (D3–D14) followed by BMP-2 (D15–D28). Differential expression of 84 genes implicated in chondrogenic and osteogenic differentiation of MSCs was analyzed at D28 by real-time RT-PCR array technology. TGF-β1 alone down-regulated two genes, CD36 and cathepsin K. Sixteen genes were significantly up-regulated, notably type 2 and type 10 collagens, COMP and Sox9. The sequential combination of TGF-β1 and BMP-2 produced a similar profile with prominent expression of type 2 collagen and the alkaline phosphatase gene. Interestingly, in this in vitro condition, RUNX2 was not up-regulated, suggesting that the sequential combination of TGF-β1/BMP2 enhances the hypertrophic chondrogenic profile without turning towards the osteoblastic pathway.
